Journal
/
Neurociência
/
A Hydrophobic Tissue Clearing Method for Rat Brain Tissue
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

A Hydrophobic Tissue Clearing Method for Rat Brain Tissue

DOI:
10.3791/61821-v

•

08:24 min

•

December 23, 2020

•

, , , , ,
1Program in Behavioral Neuroscience, Department of Psychology,University of South Carolina

Capítulos

  • 00:05Introduction
  • 00:46Sample Preparation
  • 01:36Dehydration and Depigmentation
  • 03:04CTB-Cholera Toxin Subunit B Labeling and Antibody Application
  • 04:17Tissue Clearing
  • 05:02Mounting the Sample for Imaging
  • 05:59Results: Confocal Images of the Hydrophobically Cleared Tissue Samples with Fluorescent Labelled TH Neurons and CTB Markers
  • 07:39Conclusion

Summary

Tadução automática

Tadução automática

Here we present a hydrophobic tissue clearing method that allows for the viewing of target molecules as part of intact brain structures. This technique has now been validated for F344/N control and HIV-1 transgenic rats of both sexes.

Tags

Rat BrainHIV-1 Transgenic RatTranscardial PerfusionPFA FixationMethanol DehydrationDCM-methanol IncubationHydrogen Peroxide BleachingBiotin-CTB InjectionImmunostaining3D Imaging

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/61821?article_type=v

Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
check_url/pt/61821?article_type=v

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
check_url/pt/61821?article_type=v

Primer for Immunohistochemistry on Cryosectioned Rat Brain Tissue: Example Staining for Microglia and Neurons
check_url/pt/61821?article_type=v

Using Fluorescence Activated Cell Sorting to Examine Cell-Type-Specific Gene Expression in Rat Brain Tissue
check_url/pt/61821?article_type=v

Sublimation of DAN Matrix for the Detection and Visualization of Gangliosides in Rat Brain Tissue for MALDI Imaging Mass Spectrometry
check_url/pt/61821?article_type=v

A Rat Model of Mild Intrauterine Hypoperfusion with Microcoil Stenosis
check_url/pt/61821?article_type=v

Novel Passive Clearing Methods for the Rapid Production of Optical Transparency in Whole CNS Tissue
check_url/pt/61821?article_type=v

Isolation of Cerebral Capillaries from Fresh Human Brain Tissue
check_url/pt/61821?article_type=v

Preparation of Newborn Rat Brain Tissue for Ultrastructural Morphometric Analysis of Synaptic Vesicle Distribution at Nerve Terminals
check_url/pt/61821?article_type=v

Immunostaining of Whole-Mount Retinas with the CLARITY Tissue Clearing Method

Read Article