Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

Journal

Biologia

JoVE Journal
Biologia

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JoVE Journal Biologia

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

DOI:

10.3791/61855-v

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08:20 min

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November 03, 2020

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Kanut Laoharawee^2,3, Matthew J. Johnson^2,3, Walker S. Lahr^2,3, Joseph J. Peterson^2,3, Beau R. Webber^2,3, Branden S. Moriarity^2,3

¹Department of Pediatrics,University of Minnesota, ²Center for Genomic Engineering,University of Minnesota, ³Masonic Cancer Center,University of Minnesota

Capítulos

00:04Introduction
00:56Human B-cell Expansion
02:25Primary Human B-cell Engineering
05:30Results: CRISPR/Cas9-based Genome Engineering of Primary Human B Cells for Gene Knockout and Knock-in
07:33Conclusion

Summary

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Here we provide a detailed, step-by-step protocol for CRISPR/Cas9-based genome engineering of primary human B cells for gene knockout (KO) and knock-in (KI) to study biological functions of genes in B cells and the development of B-cell therapeutics.

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

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Capítulos

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