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1Department of Internal Medicine and Biomedical Research Institute,Pusan National University Hospital, 2Biomedical Research Institute,Pusan National University Hospital, 3Department of Plastic Surgery,Pusan National University Hospital, 4Department of Otorhinolaryngology – Head and Neck Surgery,Yangsan Pusan National University Hospital, 5Department of Otorhinolaryngology – Head and Neck Surgery,Pusan National University Hospital, 6Department of Pathology,Pusan National University Hospital
Capítulos
- 00:04Introduction
- 01:00Preparation for Harvesting Rat Kidneys
- 01:55Transcardial Perfusion
- 03:22Kidney Harvesting and Decellularization
- 04:37Results: Rat Kidneys Subjected to Renal Arterial Perfusion Decellularization
- 05:35Conclusion
Este protocolo introduce un método para desarrollar un andamio usando riñones de rata descelularizados. El protocolo incluye procesos de descelularización y recelularización para confirmar la biodisponibilidad. La descelularización se realiza utilizando Tritón X-100 y dodecil sulfato de sodio.
