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Study of Protein Dynamics via Neutron Spin Echo Spectroscopy
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Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

Study of Protein Dynamics via Neutron Spin Echo Spectroscopy

DOI:
10.3791/61862-v

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08:03 min

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April 13, 2022

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1NSD, Oak Ridge National Laboratory

Capítulos

  • 00:04Introduction
  • 01:17Measurement of NSE
  • 03:58NSE Data Reduction
  • 05:05NSE Data Fitting
  • 05:33Results: NSE Relaxation Spectra for IgG and MBP Proteins
  • 06:39Conclusion

Summary

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Tadução automática

The present protocol describes methods for investigating the structure and dynamics of two model proteins that have an important role in human health. The technique combines bench-top biophysical characterization with neutron spin echo spectroscopy to access the dynamics at time and length scales relevant for protein interdomain motions.

Tags

Keywords: Neutron Spin Echo SpectroscopyProtein DynamicsProtein Domain MotionsBiophysical ResearchSample PreparationSample LoadingSample AlignmentTemperature ControlData Collection

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