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JoVE Journal Neuroscience
Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration
 

Coculture of Axotomized Rat Retinal Ganglion Neurons with Olfactory Ensheathing Glia, as an In Vitro Model of Adult Axonal Regeneration

Article DOI: 10.3791/61863-v • 07:57 min • November 2nd, 2020
November 2nd, 2020 •
1,3, 1, 2, 1, 3
1Facultad de CC Experimentales, Universidad Francisco de Vitoria, 2Dept. Biomedical and Biotechnological Sciences, Section of Physiology, University of Catania, 3Dept. Anatomía, Histología y Neurociencia, Facultad de Medicina, Universidad Autónoma de Madrid

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We present an in vitro model to assess olfactory ensheathing glia (OEG) neuroregenerative capacity, after neural injury. It is based on a coculture of axotomized adult retinal ganglion neurons (RGN) on OEG monolayers and subsequent study of axonal regeneration, by analyzing RGN axonal and somatodendritic markers.

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Keywords: Axotomized Retinal Ganglion Neurons Olfactory Ensheathing Glia In Vitro Axonal Regeneration Coculture Quantitative Technique Cell Therapy Nervous System Injuries Retinal Dissection Papain DNase Centrifugation Ovo Mucoid Protease Inhibitor NB-B27 Medium PLL-treated Coverslips OEG Monolayer
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