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卵巢组织细菌的特征和功能预测

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Characterization and Functional Prediction of Bacteria in Ovarian Tissues

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卵巢组织细菌的特征和功能预测

DOI:

10.3791/61878-v

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10:12 min

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October 23, 2021

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Lanbo Zhao*¹, Weichu Zhao*², Qi Wang*¹, Dongxin Liang, Yu Liu, Guoxing Fu, Lu Han, Yiran Wang, Chao Sun, Qing Wang, Qing Song, Qiling Li, Qinrui Lu

¹Department of Obstetrics and Gynecology,First Affiliated Hospital of Xi’an Jiaotong University, ²School of Social Sciences,University of Manchester, ³Department of Pathology,First Affiliated Hospital of Xi’an Jiaotong University, ⁴Omega Bioservices Inc

Capítulos

00:03Introduction
01:10Collect Samples
01:47Perform Bacterial LPS’s: Immunohistochemistry in Ovaries
03:50Detect 16S rRNA Sequencing
05:0216S rRNA Sequencing Analysis
06:09Predict Bacteria Function
06:36Data
07:09Representative Result
09:47Conclusion

Summary

Tadução automática

为了发现和区分原位癌和非癌卵巢组织的细菌，进行了免疫造血染色和16S核糖核化核糖核素（16S rRNA基因）测序。通过利用"未观测状态"（PICRUST）重建对社区的虫基和邻苯二甲酸酯遗传学调查来预测细菌的组成和功能差异。

Tags

Keywords: Bacteria Immunohistochemistry 16S RRNA Sequencing Characterization Functional Prediction Cancer Non-cancer BoPs Cellular-genetic Investigation In Situ Paraffin Embedding Antigen Retrieval Antibody Staining DAP Substrate HRP Polymer Dehydration 16S RRNA Sequencing Library Preparation

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