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Biologia do Desenvolvimento
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Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

Egg Microinjection and Efficient Mating for Genome Editing in the Firebrat Thermobia domestica

DOI:
10.3791/61885-v

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06:08 min

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October 20, 2020

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, , ,
1Department of Applied Biosciences, Graduate School of Agriculture,Kyoto University

Capítulos

  • 00:04Introduction
  • 00:41Egg Collection and Microinjection
  • 03:04Mating
  • 03:42Genotyping
  • 04:31Results: Dry Injection of T. domestica Eggs
  • 05:37Conclusion

Summary

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We provide a detailed protocol for rearing, microinjection of eggs and for efficient mating of the firebrat Thermobia domestica to generate and maintain mutant strains after genome editing.

Tags

Thermobia DomesticaFirebratEgg MicroinjectionGenome EditingInsect ModelCulture MaintenanceRNAiTransgenesisLaboratory Model

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