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Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
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JoVE Journal Bioengenharia
Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Single-Cell Optical Action Potential Measurement in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes

DOI:
10.3791/61890-v

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08:39 min

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December 22, 2020

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, ,
1Institute of Pharmacology and Toxicology,University Medical Center Goettingen, Goettingen, Germany, 2DZHK (German Center for Cardiovascular Research),Partner Site Goettingen, Germany, 3Cairn Research Ltd, Faversham, United Kingdom, 4Cluster of Excellence “Multiscale Bioimaging: from Molecular Machines to Networks of Excitable Cells” (MBExC),University of Goettingen, Germany

Capítulos

  • 00:05Introduction
  • 00:45Cell Preparation
  • 01:35Microscope Setup
  • 03:28Voltage Sensitive Dye Cell Loading
  • 04:24Electrical Field Stimulation
  • 04:59Optical Action Potential (AP) Acquisition
  • 06:01Data Analysis
  • 06:55Results: Representative Optical AP Profiles of Isolated Native Murine and Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes
  • 07:59Conclusion

Summary

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Here we describe optical acquisition and characterization of action potentials from induced pluripotent stem cell derived cardiomyocytes using a high-speed modular photometry system.

Tags

Keywords: Single-cellOptical Action PotentialInduced Pluripotent Stem Cell-derived CardiomyocytesPatch ClampHigh ThroughputToxicity ScreeningComprehensive In Vitro Proarrhythmia AssayVoltage Sensitive Dye ImagingEpifluorescence MicroscopeLEDPMT DetectorData Acquisition

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