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Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

Optimizing the Growth of Endothiapepsin Crystals for Serial Crystallography Experiments

DOI:
10.3791/61896-v

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09:52 min

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February 04, 2021

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,
1Swiss Light Source,Paul Scherrer Institut

Capítulos

  • 00:04Introduction
  • 00:44Preparing a Seed Stock of Endothiapepsin Crystals
  • 01:49Morphogram Experiment
  • 03:14Morphogram Analysis
  • 04:06Scaling
  • 05:56Seeded‐Batch Protocol for 20 μm Endothiapepsin Crystals
  • 07:11Results: Representative Endothiapepsin Crystal Growth and Analysis
  • 09:16Conclusion

Summary

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The aim of this article is to give the viewer a solid understanding of how to transform their small-volume, vapor-diffusion protocol, for growing large, single protein crystals, into a large-volume batch micro-crystallization method for serial crystallography.

Tags

Keywords: EndothiapepsinSerial CrystallographyMicrocrystalline SamplesVapor DiffusionCrystal Growth OptimizationFormulatrix RobotPEG 6000Tris-HClMagnesium ChlorideSodium AcetateSeed Stock

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