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中子晶体学数据收集和处理，用于蛋白质结构中氢原子的建模

JoVE Journal
Química

This content is Free Access.

JoVE Journal Química

Neutron Crystallography Data Collection and Processing for Modelling Hydrogen Atoms in Protein Structures

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中子晶体学数据收集和处理，用于蛋白质结构中氢原子的建模

DOI:

10.3791/61903-v

•

10:10 min

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December 01, 2020

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Gabriela C. Schröder², Flora Meilleur²

¹Department of Molecular and Structural Biochemistry,North Carolina State University, ²Neutron Scattering Division,Oak Ridge National Laboratory

Capítulos

00:04Introduction
00:56Crystal Harvesting and Mounting
02:28Room Temperature Data Collection
03:31Cryo Data Collection
04:36Structure Refinement
06:30Model Building
07:45Results: Representative Neutron Crystallography Data Collection and Processing
09:26Conclusion

Summary

Tadução automática

中子蛋白晶体学是一种结构技术，允许氢原子的定位，从而提供蛋白质功能的重要机制细节。我们在这里介绍了用于安装蛋白质晶体，中子衍射数据收集，结构细化以及中子散射长度密度图分析的工作流程。

Tags

Neutron Crystallography Protein Structure Hydrogen Atoms Protonation States Water Molecule Orientations Reaction Mechanisms Binding Interactions Radiation Damage Crystal Harvesting Crystal Mounting Quartz Capillary Deuterated Buffer Beeswax IMAGINE Neutron Diffractometer Goniometer Sample Stick Data Acquisition

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