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为光遗传学实验构建简单通用的照明系统
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
Building a Simple and Versatile Illumination System for Optogenetic Experiments
DOI:

06:41 min

January 12, 2021

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Capítulos

  • 00:05Introduction
  • 00:31Electrical Circuit Construction
  • 01:38Light Emitting Diode (LED) Construction
  • 05:10Results: Representative LED-Controlled Phytochrome B (PhyB) Expression Analysis
  • 06:06Conclusion

Summary

Tadução automática

该协议描述了如何使用PhyB和PIF3进行光遗传学实验以控制红光和远红光的基因表达。其中包括构建简单灵活的照明系统的分步说明,该系统可以使用计算机控制基因表达或其他光遗传学。

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