00:46Design and Fabrication of the Multichannel, Optically Transparent, Electrotactic Chip and Coating Poly-L-lysine on the Substrate in the Cell Culture Regions
02:49Coating Poly-L-lysine on the Substrate in the Cell Culture Regions and Preparation of the Salt Bridge Network
04:47Setup of the Microfluidic System for DC Pulse Stimulation
05:55Results: Differentiation of the mNPC Cells in the Control Group and in the DC Pulse Stimulation Group at DIV 3, 7, and 14
In dieser Studie stellen wir ein Protokoll zur Differenzierung von neuronalen Stamm- und Vorläuferzellen (NPCs) vor, das ausschließlich durch Gleichstrom-Pulsstimulation (DC) in einem mikrofluidischen System induziert wird.