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Xenopus laevis Ei-Extrakt-Zubereitung und Live-Bildgebungsverfahren zur Visualisierung der dynamischen zytoplasmatischen Organisation
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Biologia
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JoVE Journal Biologia
Xenopus laevis Egg Extract Preparation and Live Imaging Methods for Visualizing Dynamic Cytoplasmic Organization
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Xenopus laevis Ei-Extrakt-Zubereitung und Live-Bildgebungsverfahren zur Visualisierung der dynamischen zytoplasmatischen Organisation

DOI:
10.3791/61923-v

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05:58 min

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June 06, 2021

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,
1Department of Chemical and Systems Biology,Stanford University School of Medicine, 2Current Address: Department of Biological Sciences,University of Southern California, 3Department of Biochemistry,Stanford University School of Medicine

Capítulos

  • 00:05Introduction
  • 00:35Preparation of Slides and Coverslips
  • 01:06Preparation and Live Imaging of Interphase-Arrested Egg Extracts
  • 04:06Result: Microscopy Analysis of Interphase-Arrested Egg Extracts Self-Organized into Cell-Like Compartments
  • 05:23Conclusion

Summary

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Wir beschreiben ein Verfahren zur Herstellung und Live-Bildgebung von unverdünnten zytoplasmatischen Extrakten aus Xenopus laevis-Eiern .

Tags

Keywords: Xenopus LaevisEgg ExtractLive ImagingCytoplasmic OrganizationFEP TapeImaging SpacerDejellying SolutionMMR BufferEgg Lysis BufferCentrifugationAprotininLeupeptinCytochalasin BCyclohexamideCytoplasmic Layer

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