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Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator
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JoVE Journal Genética
Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

Embryo Injections for CRISPR-Mediated Mutagenesis in the Ant Harpegnathos saltator

DOI:
10.3791/61930-v

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08:30 min

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February 09, 2021

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, , , , ,
1Department of Biology,University of Florida, 2Department of Biochemistry and Molecular Pharmacology,New York University School of Medicine, 3Department of Radiation Oncology,Columbia University Medical Center, 4Center for Smell and Taste,University of Florida

Capítulos

  • 00:05Introduction
  • 00:40Regular Maintenance of Harpegnathos saltator Colonies
  • 02:05Preparation of Quartz Glass Microinjection Needles and Microinjector
  • 04:10Injection of Embryos
  • 06:32Results: Mutagenesis Induction in H. saltator Ants
  • 07:37Conclusion

Summary

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Tadução automática

Many characteristics of insect eusociality rely on within-colony communication and division of labor. Genetic manipulation of key regulatory genes in ant embryos via microinjection and CRISPR-mediated mutagenesis provides insights into the nature of altruistic behavior in eusocial insects.

Tags

CRISPRMutagenesisHarpegnathos SaltatorAntGenetic ModificationEmbryo InjectionMicroinjectionPlaster Nest BoxEusocial InsectsComportamentoCommunicationPhenotype PlasticityGraduate StudentAnt RearingColony MaintenanceWaste DisposalGlass Microinjection NeedlesCasein ProteinsSmall Guide RNAs

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