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使用DNA对细胞进行简单、经济、模块化的图案化
JoVE Journal
Bioengenharia
This content is Free Access.
JoVE Journal Bioengenharia
Simple, Affordable, and Modular Patterning of Cells using DNA
DOI:

08:59 min

February 24, 2021

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Capítulos

  • 00:05Introduction
  • 00:30Photopattern DNA onto Aldehyde-Functionalized Slides
  • 02:46Lift and Label Cells with Cholesterol-Modified DNA
  • 04:21Pattern the DNA-labeled Cells and Transferring into Hydrogel for 3D Culture
  • 07:22Results: Adhesion of CMO-Labeled Cells to DNA Patterns
  • 08:32Conclusion

Summary

Tadução automática

在这里,我们提出了一种使用DNA程序化粘附的单细胞分辨率的微模式细胞方案。该协议使用台式光刻平台在载玻片上创建DNA寡核苷酸的图案,然后用市售的互补寡核苷酸标记细胞膜。寡核苷酸的杂交导致程序化的细胞粘附

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