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A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells
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Bioengenharia
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JoVE Journal Bioengenharia
A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

A Culture Method to Maintain Quiescent Human Hematopoietic Stem Cells

DOI:
10.3791/61938-v

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07:14 min

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May 17, 2021

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1Department of Stem Cell Biology, Research Institute,National Center for Global Health and Medicine

Capítulos

  • 00:04Introduction
  • 01:16Lipid Preparation
  • 02:13Media Preparation
  • 03:43Cell Culture
  • 04:47Results: Cell Numbers After 7 Days of Culturing
  • 06:20Conclusion

Summary

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This protocol enables the maintenance of quiescent human hematopoietic stem cells in vitro by mimicking the bone marrow microenvironment using abundant fatty acids, cholesterol, lower concentrations of cytokines, and hypoxia.

Tags

Keywords: Hematopoietic Stem CellsQuiescenceCell CycleIn Vitro CultureAnti-cancer DrugsLipid SolutionCulture MediaStem Cell FactorThrombopoietin

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