3D细胞打印缺氧肿瘤芯片用于模拟实体癌的病理进展
4.5K 次观看
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被引用 7 次
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10:51 分钟
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2021年1月5日
DOI :10.3791/61945-v
2021年1月5日
•4.5K 次观看
1Department of Mechanical Engineering, Pohang University of Science and Technology (POSTECH), 2Department of Creative IT Engineering, Pohang University of Science and Technology (POSTECH), 3Department of Rural and Biosystems Engineering, College of Agriculture and Life Sciences, Chonnam National University
* These authors contributed equally
缺氧是肿瘤微环境的显著特征，在癌症进展中发挥关键作用。本文介绍了一种基于3D细胞打印技术的缺氧肿瘤芯片的制备过程，用于模拟与缺氧相关的癌症病理特征。
Chapters in this video
0:05
Introduction
0:56
Collagen Pre-Gel Solution Preparation
1:47
1% Neutralized Collagen Pre-Gel Solution pH Adjustment
3:12
Gas-Permeable Barrier 3D Printing
4:20
Polydimethylsiloxane (PDMS) Barrier Casting
5:29
1% Neutralized Collagen Pre-Gel Solution Cell Mixing
6:19
Cancer-Stroma Concentric Ring 3D Cell Printing
7:39
Hypoxic Cancer-on-a-Chip Maintenance
8:11
Results: Representative Solid Pathologic Cancer Progression on a 3D Cell-Printed Hypoxic Cancer-on-a-Chip
10:06
Conclusion
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