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In vitro Time-lapse Live-Cell Imaging to Explore Cell Migration toward the Organ of Corti
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JoVE Journal Medicina
In vitro Time-lapse Live-Cell Imaging to Explore Cell Migration toward the Organ of Corti

In vitro Time-lapse Live-Cell Imaging to Explore Cell Migration toward the Organ of Corti

DOI:
10.3791/61947-v

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08:08 min

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December 04, 2020

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, , , ,
1Department of Otorhinolaryngology,Yonsei University Wonju College of Medicine, 2Research Institute of Hearing Enhancement,Yonsei University Wonju College of Medicine, 3Department of Otorhinolaryngology Head and Neck Surgery,Hallym University College of Medicine

Capítulos

  • 00:04Introduction
  • 01:14Cochleae Dissection
  • 04:04Time-lapse Imaging
  • 05:58Results: Confocal Images of the Organ of Corti and GFP-labeled MSCs
  • 07:26Conclusion

Summary

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In this study, we present a real-time imaging method using confocal microscopy to observe cells moving toward damaged tissue by ex vivo incubation with the cochlear epithelium containing the organ of Corti.

Tags

Keywords: In VitroTime-lapseLive-cell ImagingCell MigrationOrgan Of CortiMesenchymal Stem Cells (MSCs)TranswellWound HealingTectorial MembraneCochleaOrgan Culture

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