Journal
/
/
胎児および成人神経幹細胞由来オリゴデンドロビテ前駆細胞培養の高含有スクリーニング分化と成熟解析
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
High-Content Screening Differentiation and Maturation Analysis of Fetal and Adult Neural Stem Cell-Derived Oligodendrocyte Precursor Cell Cultures
DOI:

09:36 min

March 10, 2021

Capítulos

  • 00:04Introduction
  • 00:48Fetal Neural Stem Cell (NSC) Dissection and Isolation
  • 02:18Adult NSC Culture
  • 04:05Primary Neurosphere, Oligosphere, and Oligodendrocyte Precursor Cell (OPC) Differentiation
  • 05:05Inflammation-Mediated Differentiation Block and Oxygen-Glucose Deprivation (OGD) Cell Death Induction
  • 05:56High-Content Screening (HCS) Cell Viability, Lineage Composition, and Lineage-Specific Cell Death Analyses
  • 07:18Results: Representative Oligosphere Differentiation Characterization
  • 09:04Conclusion

Summary

Tadução automática

成熟したオリゴデンドロサイトに分化する胎児または成体の神経幹細胞に由来するアストロサイトとオリゴデンドロサイト前駆細胞の混合培養の生成と、有害刺激のインビトロモデリングについて述べています。細胞ベースの高含有スクリーニング技術との結合は信頼でき、強い薬物スクリーニングシステムを造る。

Vídeos Relacionados

Read Article