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Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System
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Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

Modeling Breast Cancer in Human Breast Tissue using a Microphysiological System

DOI:
10.3791/62009-v

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10:51 min

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April 23, 2021

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, , , , , ,
1Department of Surgery,Louisiana State University Health Sciences Center, 2Department of Bioinnovation,Tulane University, 3Tulane University School of Medicine, 4Department of Biological and Agricultural Engineering,Louisiana State University, 5Department of Medicine,Tulane University School of Medicine

Capítulos

  • 00:04Introduction
  • 01:10Preparation of Gelatin Plungers and Application to the Upper Cell Sheets
  • 02:21Processing of Human Breast Tissue
  • 05:21Digestion of BC-MPS for Analysis
  • 08:09Results: Analysis of BC-MPS Culture
  • 10:00Conclusion

Summary

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This protocol describes the construction of an in vitro microphysiological system for studying breast cancer using primary human breast tissue with off the shelf materials.

Tags

Breast CancerMicrophysiological SystemEx VivoAdipocytesImmune CellsVascular StructuresDuctal StructuresExtracellular MatrixPersonalized MedicinePharmaceutical DevelopmentTumor InitiationFibrosisExtracellular Matrix RemodelingGelatinPNIPAAmASCsPBSRazor BladeCancer Cell LinesBC-MPS Media

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