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Kemisk bøjning af et renset DEC-205-rettet antistof med protein i fuld længde til målretning af musededritiske celler In Vitro og In Vivo
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1Immune Regulation Group,Helmholtz Centre for Infection Research, 2Infection Immunology Group, Institute of Medical Microbiology, Infection Prevention and Control, Health Campus Immunology, Infectiology and Inflammation,Otto-von-Guericke University Magdeburg, 3Institute for Molecular and Clinical Immunology, Health Campus Immunology, Infectiology and Inflammation,Otto-von-Guericke University Magdeburg, 4Cellular Proteome Research,Helmholtz Centre for Infection Research, 5Experimental Pneumology, University Hospital of Pneumology, Health Campus Immunology, Infectiology and Inflammation,Otto-von-Guericke University Magdeburg
Capítulos
- 00:04Introduction
- 00:50Anti-DEC-205 (CD205) Production
- 02:47Anti-DEC-205 Antibody Purification
- 04:40Chemical Conjugation
- 06:43Chemical Conjugation Verification
- 08:22Results: Representative Purified anti-DEC-205 Antibody Conjugation Characterization and Analysis
- 09:52Conclusion
Vi beskriver en protokol for den kemiske bøjning af modellen antigen ovalbumin til en endokytose receptor-specifikke antistof for in vivo dendritiske celle målretning. Protokollen omfatter rensning af antistoffet, kemisk bøjning af antigen, samt rensning af konjugaten og verifikation af effektiv bøjning.
