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Biologia do Desenvolvimento
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Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

Separation of Follicular Cells and Oocytes in Ovarian Follicles of Zebrafish

DOI:
10.3791/62027-v

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11:35 min

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April 18, 2021

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, , , , ,
1College of Life Sciences, Northwest Normal University, 2State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology, Chinese Academy of Sciences, 3Department of Bioscience,Tokyo University of Agriculture

Capítulos

  • 00:00Introduction
  • 02:16Preparation of Pulled Glass Capillary
  • 03:18Experimental Preparation
  • 04:40Separation of Zebrafish Oocytes and Follicular Cells at Different Stages
  • 08:08Resultados
  • 10:12Conclusion

Summary

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Here, we present a simple method for separating follicular cells and oocytes in zebrafish ovarian follicles, which will facilitate investigations of ovarian development in zebrafish.

Tags

Keywords: Follicular CellsOocytesOvarian FolliclesZebrafishSeparationDissectionGlass CapillaryIn Vitro FertilizationOvaryOvarian Development

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