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A Step-by-Step Guide to Mosquito Electroantennography
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Biologia
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JoVE Journal Biologia
A Step-by-Step Guide to Mosquito Electroantennography

A Step-by-Step Guide to Mosquito Electroantennography

DOI:
10.3791/62042-v

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06:39 min

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March 10, 2021

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1Department of Biochemistry; The Fralin Life Science Institute; The Global Change Center; Department of Entomology and the Center for Emerging Zoonotic and Arthropod-Borne Pathogens,Virginia Polytechnic Institute and State University

Capítulos

  • 00:05Introduction
  • 00:37Odor Storage and Electrode Holder-Capillary Preparation
  • 02:22Electroantennography (EAG) Rig Preparation
  • 02:57Mosquito Head Preparation and Mounting
  • 04:10Recordings and Data Analysis
  • 04:47Results: Olfactory Mediated Behaviors of Mosquito Species from Various Genera
  • 06:01Conclusion

Summary

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The present article details a step-by-step protocol for successful and low noise electroantennograms in several genera of mosquitoes, including both females and males.

Tags

Keywords: ElectroantennographyMosquitoesPhysiological StatusInsect ModelsOdorant MixturesBorosilicate CapillariesChloridizationSaline SolutionReference ElectrodeRecording ElectrodeAir TableMedical AirHumidifierControl PulseAluminum PlateWet Cleaning Wipe

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