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通过使用来自辐照的Buffy外套的饲养细胞的T淋巴细胞的分离和培养来研究主动脉瓣钙化

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Investigating Aortic Valve Calcification via Isolation and Culture of T Lymphocytes using Feeder Cells from Irradiated Buffy Coat

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通过使用来自辐照的Buffy外套的饲养细胞的T淋巴细胞的分离和培养来研究主动脉瓣钙化

DOI:

10.3791/62059-v

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04:30 min

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February 04, 2021

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Lavinia Curini*^1,2, Mary Roxana Christopher*¹, Herko Grubitzsch⁴, Ulf Landmesser³, Amedeo Amedei*^2,5, Alexander Lauten*^1,6, Brunilda Alushi*^1,6

¹Department of Cardiology, Campus Benjamin Franklin,Charité Universitätsmedizin Berlin and German Centre for Cardiovascular Research (DZHK), ²Department of Experimental and Clinical Medicine,University of Florence, ³Berlin Institute of Health, ⁴Department of Cardiology,German Heart Centre Berlin (DHZB), ⁵Sod of Interdisciplinary Internal Medicine,Azienda Ospedaliera Universitaria Careggi (AOUC), ⁶Department of General and Interventional Cardiology,Helios Klinikum Erfurt

Capítulos

00:05Introduction
00:30Cloning Phase
01:48T Lymphocyte Culture
02:52Results: Representative Calcified Aortic Valve T Cell Isolation and Analysis
03:52Conclusion

Summary

Tadução automática

在本研究中，我们描述了从钙化主动脉瓣新鲜样品中分离T淋巴细胞的过程，以及使用流式细胞术分析表征适应性白细胞亚群的T细胞克隆的分析步骤。

Tags

T Lymphocytes Aortic Valve Calcification Feeder Cells Density Gradient Centrifugation Cell Culture PBMC Isolation IL-2 T-cell Cloning T-cell Culture U-bottom 96-well Plates

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