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Visualisering af SARS-CoV-2 ved hjælp af Immuno RNA-fluorescens In Situ Hybridisering
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1Malopolska Centre of Biotechnology,Jagiellonian University, 2Microbiology Department Faculty of Biochemistry, Biophysics and Biotechnology,Jagiellonian University, 3Department of Cardiac, Vascular and Endovascular Surgery and Transplantology,The Medical University of Silesia in Katowice, 4Silesian Centre for Heart Diseases
Capítulos
- 00:04Introduction
- 00:42SARS-CoV-2 RNA-FIS in Vero Cells Cultured on Coverslips
- 02:26SARS-CoV-2 RNA-FISH in HAE Cultures
- 03:38Results: Immuno-RNA-FISH to Detect SARS-CoV-2 RNA in Vero and HAE Cells
- 04:38Conclusion
Her beskriver vi en simpel metode, der kombinerer RNA-fluorescens in situ-hybridisering (RNA-FISH) med immunfluorescens for at visualisere svær akut respiratorisk syndrom coronavirus 2 (SARS-CoV-2) RNA. Denne protokol kan øge forståelsen af de molekylære egenskaber ved SARS-CoV-2 RNA-værtsinteraktioner på et enkeltcellet niveau.
