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检查斑马鱼肌肉疾病模型中的肌肉再生
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Examining Muscle Regeneration in Zebrafish Models of Muscle Disease
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检查斑马鱼肌肉疾病模型中的肌肉再生

DOI:
10.3791/62071-v

•

07:58 min

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January 18, 2021

•

, ,
1Australian Regenerative Medicine Institute,Monash University

Capítulos

  • 00:04Introduction
  • 00:41Live Embryo Genotyping
  • 02:58Muscle Injury
  • 03:55Muscle Regeneration Quantification
  • 05:54Results: Representative Muscle Regeneration Analysis in Wildtype and Lama2 Deficient Zebrafish Larvae
  • 07:10Conclusion

Summary

Tadução automática

Tadução automática

骨骼肌再生是由组织常驻肌肉干细胞驱动的，这些干细胞在许多肌肉疾病（如肌肉萎缩症）中受损，这导致肌肉无法再生。在这里，我们描述了一个协议，允许检查肌肉再生的斑马鱼模型的肌肉疾病。

Tags

Keywords: ZebrafishMuscle RegenerationMuscle DiseaseHigh-throughputIn VivoGenotypingDNA ExtractionEmbryoDownstream Genotyping AssaysMuscle Injury

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