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Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy
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Bioquímica
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JoVE Journal Bioquímica
Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

DOI:
10.3791/62079-v

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06:18 min

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April 23, 2021

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, , ,
1Department of Nephrology, Medical Faculty,Heinrich-Heine-University

Capítulos

  • 00:05Introduction
  • 00:27Preparation for Expansion Microscopy
  • 01:24Anchoring and Polymerization (gelation)
  • 02:54Homogenization (digestion)
  • 04:14Expansion and Imaging
  • 05:04Results: Podocin Co-localizes with Nephrin and Actin
  • 05:46Conclusion

Summary

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The presented method enables visualization of fluorescently labeled cellular proteins with expansion microscopy leading to a resolution of 70 nm on a conventional microscope.

Tags

Expansion MicroscopyPodocyteNephrinActinPodocinGelation ChamberAnchoring TreatmentPhalloidinSodium AcrylateMonomer SolutionAPSGel Polymerization

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