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JoVE Journal Biochemistry
Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy
 

Imaging of Podocytic Proteins Nephrin, Actin, and Podocin with Expansion Microscopy

Article DOI: 10.3791/62079-v • 06:18 min • April 23rd, 2021
April 23rd, 2021 •
*1, *1, 1, 1
1Department of Nephrology, Medical Faculty, Heinrich-Heine-University
* These authors contributed equally

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Resumo
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The presented method enables visualization of fluorescently labeled cellular proteins with expansion microscopy leading to a resolution of 70 nm on a conventional microscope.

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Keywords: Expansion Microscopy Podocyte Nephrin Actin Podocin Gelation Chamber Anchoring Treatment Phalloidin Sodium Acrylate Monomer Solution APS Gel Polymerization
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