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Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

Systematic Scoring Analysis for Intestinal Inflammation in a Murine Dextran Sodium Sulfate-Induced Colitis Model

DOI:
10.3791/62135-v

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09:11 min

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February 14, 2021

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, , , , ,
1Department of Pathology,University of Michigan, 2Department of Surgery, Klinikum Rechts der Isar,Technische Universität München, 3Wallenberg Centre for Molecular Medicine,Linköping University, 4Department of Biomedical and Clinical Sciences (BKV),Linköping University

Capítulos

  • 00:04Introduction
  • 00:50Tissue Harvest
  • 02:24Preparation of Swiss Rolls
  • 03:57Digital Scanning and Analysis
  • 06:33Results: Histological Colitis Score and Percentage of Injury and Ulceration Correlates with Body Weight and Stool Index During DSS Experimental Colitis
  • 08:30Conclusion

Summary

Tadução automática

Tadução automática

Systematic scoring of intestinal inflammation using a free computer-assisted system is a powerful tool to quantitatively compare histopathological changes in colitis models characterized by the presence of ulcers and inflammatory changes. Histological colitis score evaluation strengthens clinical observations and facilitates data interpretation.

Tags

Keyword Extraction: Systematic Scoring AnalysisIntestinal InflammationMurine Dextran Sodium Sulfate-induced Colitis ModelMucosal InjuryHistological InterpretationQuantitative AnalysisIntestinesMiceColitisEuthanized MouseDissecting PadAbdominal SkinPeritoneumLarge IntestinePelvic BoneColonPBSWestern BlotPCRImmunofluorescence MicroscopyNeutral Buffered Formalin Solution

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