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Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

Platform Incubator with Movable XY Stage: A New Platform for Implementing In-Cell Fast Photochemical Oxidation of Proteins

DOI:
10.3791/62153-v

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05:50 min

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May 17, 2021

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, , , ,
1Department of Pharmaceutical Sciences,University of Maryland Baltimore, 2Technology Research Center,University of Maryland Baltimore County, 3Department of Biochemistry and Molecular Biology,University of Massachusetts

Capítulos

  • 00:04Introduction
  • 00:45Setting Up the Platform Incubator for IC-FPOP
  • 02:08IC-FPOP in the Platform Incubator
  • 03:41Results: Analysis of Cell Transfection Efficiency, Proteins Modified in Single Cell Flow-System and PIXY, and Localization of IC-FPOP Modifications
  • 05:22Conclusion

Summary

Tadução automática

Tadução automática

A new static platform is used to characterize protein structure and interaction sites in the native cell environment utilizing a protein footprinting technique called in-cell fast photochemical oxidation of proteins (IC-FPOP).

Tags

Keywords: Platform IncubatorMovable XY StageIn-cell FPOPProtein-ligand InteractionsProtein-protein InteractionsConformational ChangesAutomated Six-well PlateCell CultureHydrogen PeroxideQuench BufferPeristaltic PumpsLaserCell ScraperCentrifugation

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