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Imunologia e Infecção
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Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

Ubiquitous and Tissue-specific RNA Targeting in Drosophila Melanogaster using CRISPR/CasRx

DOI:
10.3791/62154-v

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06:37 min

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February 05, 2021

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, , , ,
1Division of Biological Sciences, Section of Cell and Developmental Biology,University of California

Capítulos

  • 00:05Introduction
  • 00:32Ubiquitous In Vivo RNA Targeting Using a Two-Component CasRx System
  • 01:52Ubiquitous In Vivo Exogenous RNA Targeting Using a Three-Component CasRx System
  • 04:13Luciferase Assay
  • 04:53Results: Representative Ubiquitous and Tissue-Specific RNA Targeting in Drosophila melanogaster
  • 06:13Conclusion

Summary

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This article outlines a detailed protocol for using the RNA-targeting Cas13D enzyme (RfxCas13D) in flies.

Tags

CRISPRCasRxRNA TargetingDrosophila MelanogasterUbiquitousGene Transcript ReductionTwo-component SystemThree-component SystemGRNAUbiq CasRxLuciferasePhenotypeMendelian Genetics

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