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Biologia do Desenvolvimento
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Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

DOI:
10.3791/62163-v

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06:56 min

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January 21, 2021

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, , ,
1Regenerative Bioscience Center; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences,University of Georgia

Capítulos

  • 00:04Introduction
  • 00:37Separation of the Tongue Epithelium from the Mesenchyme/Underlying Connective Tissue
  • 03:51Cell Dissociation
  • 05:06Results: Effect of Cell Dissociation on Total Cell Number and Viability
  • 06:29Conclusion

Summary

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We have developed a generalized protocol to dissociate a large quantity of high-quality single cells from the epithelium and mesenchyme/connective tissue of embryonic and adult mouse tongues.

Tags

Cell DissociationTongue EpitheliumTongue MesenchymeEmbryonic Day 12.58-week-old MiceSingle Cell RNA SequencingPrimary Stem Cell CulturesEnzymatic DigestionTyrode’s SolutionDispaseCollagenase

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