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从舌头上皮和梅森奇姆/胚胎日12.5和8周大的老鼠的结缔细胞分离

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Cell Dissociation from the Tongue Epithelium and Mesenchyme/Connective Tissue of Embryonic-Day 12.5 and 8-Week-Old Mice

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从舌头上皮和梅森奇姆/胚胎日12.5和8周大的老鼠的结缔细胞分离

DOI:

10.3791/62163-v

•

06:56 min

•

January 21, 2021

•

Wenxin Yu, Mohamed Ishan, Zhonghou Wang, Hong-Xiang Liu

¹Regenerative Bioscience Center; Department of Animal and Dairy Science, College of Agricultural and Environmental Sciences,University of Georgia

Capítulos

00:04Introduction
00:37Separation of the Tongue Epithelium from the Mesenchyme/Underlying Connective Tissue
03:51Cell Dissociation
05:06Results: Effect of Cell Dissociation on Total Cell Number and Viability
06:29Conclusion

Summary

Tadução automática

我们开发了一个通用的协议，将大量高质量的单细胞从胚胎和成年小鼠舌头的上皮和间质/结缔组织分离出来。

Tags

Cell Dissociation Tongue Epithelium Tongue Mesenchyme Embryonic Day 12.5 8-week-old Mice Single Cell RNA Sequencing Primary Stem Cell Cultures Enzymatic Digestion Tyrode’s Solution Dispase Collagenase

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