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Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes
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JoVE Journal Bioquímica
Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

Fluorescence-Based Measurements of Phosphatidylserine/Phosphatidylinositol 4-Phosphate Exchange Between Membranes

DOI:
10.3791/62177-v

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08:49 min

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March 14, 2021

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, , ,
1Université Côte d’Azur, Centre National de la Recherche Scientifique, Institut de Pharmacologie Moléculaire et Cellulaire

Capítulos

  • 00:04Introduction
  • 00:59Measurement of PS or PI(4)P Extraction
  • 02:44Real-time Measurement of PS Transport
  • 04:54Real-time Measurement of PI(4)P Transport
  • 06:23Results: Membrane-Exchange of Phosphatidylserine/ Phosphatidylinositol 4-Phosphate Using Fluorescent Sensors
  • 07:58Conclusion

Summary

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Here, we describe protocols using fluorescent lipid sensors and liposomes to determine whether a protein extracts and transports phosphatidylserine or phosphatidylinositol 4-phosphate in vitro.

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Keywords: FluorescencePhosphatidylserinePhosphatidylinositol 4-phosphateLipid TransferMembraneLiposomesNBD-C2 LactLipid SensorLipid Transfer ProteinNBD-PH FAPP ProbePI(4)P Extraction AssayHEPES Potassium Acetate BufferMagnesium ChloridePhosphatidylcholinePhosphatidylethanolamine

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