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Functional Characterization of Endogenously Expressed Human RYR1 Variants
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Medicina
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JoVE Journal Medicina
Functional Characterization of Endogenously Expressed Human RYR1 Variants

Functional Characterization of Endogenously Expressed Human RYR1 Variants

DOI:
10.3791/62196-v

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07:59 min

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June 09, 2021

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, ,
1Department of Biomedicine,Basel University Hospital, 2Department of Life Sciences,University of Ferrara, 3Department of Anesthesia,Basel University Hospital

Capítulos

  • 00:04Introduction
  • 01:00Intracellular Cell Culture Ca2+ Measurement
  • 02:30Intracellular Single Cell Ca2+ Measurement
  • 03:56Human Myotube Preparation
  • 04:40Human Myotube Inducible Calcium ([Ca2+]i) Measurement
  • 05:33Results: Representative Single Cell Ca2+ Release in EBV-Immortalized Human B Cells and Isolated Human Myotubes
  • 07:11Conclusion

Summary

Tadução automática

Tadução automática

Here methods used to study the functional effect of RYR1 mutations endogenously expressed in Epstein Barr Virus immortalized human B-lymphocytes and muscle biopsy derived satellite cells differentiated into myotubes are described.

Tags

RYR1 VariantsCalcium HomeostasisEBV-transformed B LymphocytesFura-2 AMKrebs Ringer’s SolutionCalcium Release ResponseSingle Cell AnalysisSpectrofluorometerPerfusion ChamberFluorescent Microscopy

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