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Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy
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JoVE Journal Bioquímica
Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

Fast Grid Preparation for Time-Resolved Cryo-Electron Microscopy

DOI:
10.3791/62199-v

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10:05 min

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November 06, 2021

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, , ,
1School of Biomedical Sciences, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, 2School of Molecular and Cellular Biology, Faculty of Biological Sciences & Astbury Centre for Structural and Molecular Biology,University of Leeds, 3Department of Physiological Sciences,Eastern Virginia Medical School

Capítulos

  • 00:10Introduction
  • 00:43Preparing the System
  • 04:34Fast Grid Preparation
  • 07:12Time-Resolved Cryo-EM
  • 08:46Representative Results
  • 09:34Conclusion

Summary

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Tadução automática

Here, we provide a detailed protocol for the use of a rapid grid making device for both fast grid-making and for rapid mixing and freezing to conduct time-resolved experiments.

Tags

Keywords: Cryo-electron MicroscopyTime-resolvedGrid PreparationRapidProtein ConcentrationSyringe PumpNitrogen GasBufferSpray NozzlePlunging Arm

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