Journal
/
Bioquímica
/
Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography
JoVE Journal
Bioquímica
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Bioquímica
Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

Analysis of Neutral Lipid Synthesis in Saccharomyces cerevisiae by Metabolic Labeling and Thin Layer Chromatography

DOI:
10.3791/62201-v

•

08:30 min

•

February 02, 2021

•

,
1Department of Cell Biology,University of Texas Southwestern Medical Center

Capítulos

  • 00:04Introduction
  • 00:30Growth and Labelling of Yeast Cells
  • 02:55Isolation of Total Lipids from Yeast
  • 04:42Thin Layer Chromatography and Quantification of Results
  • 07:09Results: 14C-Acetic Acid Radiolabeling Detection Analysis of Multiple NL Species
  • 08:03Conclusion

Summary

Tadução automática

Tadução automática

Here, a protocol is presented for the metabolic labeling of yeast with 14C-acetic acid, which is coupled with thin layer chromatography for the separation of neutral lipids.

Tags

Metabolic LabelingThin Layer ChromatographyLipid MetabolismSaccharomyces CerevisiaeYeast CultureRadiolabelingQuenching BufferCentrifugationLipid SynthesisEnzyme Regulation

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification

Absolute Quantification of Cell-Free Protein Synthesis Metabolism by Reversed-Phase Liquid Chromatography-Mass Spectrometry

Quantitative Analysis of the Cellular Lipidome of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Semi-Quantitative Analysis of Peptidoglycan by Liquid Chromatography Mass Spectrometry and Bioinformatics

Utilization of Grafix for the Detection of Transient Interactors of Saccharomyces cerevisiae Spliceosome Subcomplexes

Labelling and Visualization of Mitochondrial Genome Expression Products in Baker’s Yeast Saccharomyces cerevisiae

Quantitative Metabolomics of Saccharomyces Cerevisiae Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Preparation and Cryo-FIB micromachining of Saccharomyces cerevisiae for Cryo-Electron Tomography

Assessment of Submitochondrial Protein Localization in Budding Yeast Saccharomyces cerevisiae

A Nanobar-Supported Lipid Bilayer System for the Study of Membrane Curvature Sensing Proteins in vitro

Read Article