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Generering og udvidelse af humane kardiomyocytter fra patientens perifere blod mononukleare celler
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Generation and Expansion of Human Cardiomyocytes from Patient Peripheral Blood Mononuclear Cells

Generering og udvidelse af humane kardiomyocytter fra patientens perifere blod mononukleare celler

DOI:
10.3791/62206-v

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05:38 min

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February 12, 2021

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, , , , , ,
1Center for Cardiovascular Research, The Abigail Wexner Research Institute,Nationwide Children’s Hospital, 2The Heart Center,Nationwide Children’s Hospital, 3Department of Physiology and Cell Biology,The Ohio State University College of Medicine, 4Department of Anatomy,The Ohio State University College of Medicine, 5MCDB Graduate Program,The Ohio State University, 6Department of Pediatrics,The Ohio State University College of Medicine

Capítulos

  • 00:04Introduction
  • 01:08Human Induced Pluripotent Stem Cell (iPSC) Maintenance and Passaging
  • 01:55Chemically Defined Cardiomyocyte (CM) Differentiation
  • 02:57Results: Representative iPSC-CM Differentiation and Function
  • 04:51Conclusion

Summary

Tadução automática

Tadução automática

Her præsenterer vi en protokol til robust at generere og udvide menneskelige kardiomyocytter fra patientens perifere blodmonnukleare celler.

Tags

Cardiomyocyte GenerationPeripheral Blood Mononuclear CellsIPSC ReprogrammingCardiovascular Disease ModelingCardiac Toxicity TestingDifferentiation ProtocolCell CultureSendai VirusPBMCE8 MediumCardiomyocyte Differentiation Media

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