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Imunologia e Infecção

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小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Purification and Expansion of Mouse Invariant Natural Killer T Cells for in vitro and in vivo Studies

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小鼠不变自然杀伤性T细胞的纯化和扩增用于体外和体内研究

DOI:

10.3791/62214-v

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08:37 min

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February 15, 2021

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Gloria Delfanti, Alessandra Perini*^1,2, Elisa Zappa*^1,2, Maya Fedeli²

¹Experimental Immunology Unit, Division of Immunology, Transplantation and Infectious Diseases,IRCCS San Raffaele Scientific Institute, ²Vita-Salute San Raffaele University

Capítulos

00:04Introduction
00:39Spleen Processing
01:29T Cell Enrichment
03:27iNKT Cell Enrichment
05:21iNKT Cell Activation and Expansion
06:35Results: Enrichment, Expansion, and Characterization of iNKT Cells
07:50Conclusion

Summary

Tadução automática

我们描述了一种快速而强大的方案，以从小鼠脾脏中富集不变的自然杀伤T（iNKT）细胞，并在体外将其扩增至适合体外和体内研究的数量。

Tags

Keywords: Mouse Invariant Natural Killer T Cells Purification Expansion In Vitro In Vivo Cell Isolation Cell Enrichment MACS Cell Staining Cell Separation Cell Culture

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