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iPSC-巨噬细胞死亡神经母细胞的体外定量成像检测

JoVE Journal
Neurociência

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JoVE Journal Neurociência

In vitro Quantitative Imaging Assay for Phagocytosis of Dead Neuroblastoma Cells by iPSC-Macrophages

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iPSC-巨噬细胞死亡神经母细胞的体外定量成像检测

DOI:

10.3791/62217-v

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09:48 min

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February 14, 2021

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Hazel Hall-Roberts^2,3, Elena Di Daniel, William S. James, John B. Davis, Sally A. Cowley

¹James Martin Stem Cell Facility, Sir William Dunn School of Pathology,University of Oxford, ²Alzheimer’s Research UK Oxford Drug Discovery Institute, Nuffield Department of Medicine Research Building,University of Oxford, ³UK Dementia Research Institute,Cardiff University

Capítulos

00:04Introduction
00:41Preparation of Dead SH-SY5Ys
02:03Labeling of Dead SH-SY5Ys with pH-Sensitive Red Fluorescent Dye
03:18Staining of iPSC-Macrophages
04:50Fixed-Cell High-Content Imaging
07:28Results: Imaging, Optimization and Validation of Phagocytosis Assay
09:16Conclusion

Summary

Tadução automática

神经退行性疾病与受管制的微胶质功能障碍有关。本文概述了iPSC巨噬细胞对神经母细胞的体外测定。定量显微镜读数用于活细胞延时成像和固定细胞高含量成像。

Tags

In Vitro Quantitative Imaging Assay Phagocytosis Dead Neuroblastoma Cells IPSC-macrophages Neurodegenerative Disease SH-SY5Y Cells Cell Dissociation Fixation PH-sensitive Dye Fluorescent Dye Macrophage Media Hoechst 33342

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