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A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues
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Neurociência
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JoVE Journal Neurociência
A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

A Time-Efficient Fluorescence Spectroscopy-Based Assay for Evaluating Actin Polymerization Status in Rodent and Human Brain Tissues

DOI:
10.3791/62268-v

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06:54 min

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June 03, 2021

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,
1Department of Anatomy, School of Biomedical Sciences,University of Otago

Capítulos

  • 00:00Introduction
  • 01:49Preparation of Buffers and Reagents
  • 02:02Preparation of Samples
  • 02:56KCl-mediated Depolarization of Isolated Synaptic Terminals
  • 03:21Fixation and Phalloidin Staining of Samples
  • 04:40Fluorometric Analysis and Light Scattering
  • 05:20Data Analysis
  • 06:19Conclusion

Summary

Tadução automática

Tadução automática

We report a simple, time-efficient and high-throughput fluorescence spectroscopy-based assay for the quantification of actin filaments in ex vivo biological samples from brain tissues of rodents and human subjects.

Tags

Keywords: ActinCytoskeletonF-actinG-actinPolymerizationFluorescence SpectroscopyPhalloidinSynaptic TerminalsSynaptosomesSynaptoneurosomesNeuropathologyNeuronal Physiology

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