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Biologia do Desenvolvimento
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Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

Vitrification of In Vitro Matured Oocytes Collected from Adult and Prepubertal Ovaries in Sheep

DOI:
10.3791/62272-v

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06:53 min

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July 10, 2021

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, , , ,
1Department of Veterinary Medicine,University of Sassari

Capítulos

  • 00:00Introduction
  • 01:11Protocol
  • 03:52Resultados
  • 05:38Conclusion

Summary

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The protocol aims at providing a standard method for the vitrification of adult and juvenile sheep oocytes. It includes all the steps from the preparation of the in vitro maturation media to the post-warming culture. Oocytes are vitrified at the MII stage using Cryotop to ensure the minimum essential volume.

Tags

VitrificationOocyteIn Vitro MaturationSheepPrepubertalCryopreservationTrehaloseCryotopsWarmingCytoplasmZona PellucidaPolar BodyMinimum Essential Volume Method

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