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使用 D1S80 轨迹在实验室类中应用 DNA 指纹
JoVE Journal
Genética
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This content is Free Access.
JoVE Journal Genética
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
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使用 D1S80 轨迹在实验室类中应用 DNA 指纹

DOI:
10.3791/62305-v

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08:35 min

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July 17, 2021

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, , , ,
1Institute of Plant Genetics,Heinrich-Heine-University, 2Cluster of Excellence on Plant Sciences “SMART Plants for Tomorrow’s Needs”, 3Max Planck Institute for Plant Breeding Research, 4IBG-1: Biotechnology, Forschungszentrum Jülich GmbH

Capítulos

  • 00:00Introduction
  • 01:03Step 1: DNA Template Preparation
  • 03:18Step 2: Amplification of DIS80 VNTR Locus by PCR
  • 04:02Step 3: Agarose Gel Electrophoresis of PCR Products
  • 05:02Step 4: Analysis of Fragment Length Results
  • 06:05Representative Results
  • 06:39Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们描述了一个简单的协议，通过放大VNTR轨迹 D1S80 从上皮细胞DNA生成DNA指纹配置文件。

Tags

DNA FingerprintingD1S80 LocusVariable Number Of Tandem RepeatsDNA ExtractionPCRGel ElectrophoresisBuccal Mucosa Epithelial CellsLyse SolutionDenaturation BufferIsopropanolEthanolElution BufferUndergraduate Lab Class

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