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海洋試料における総脂質および脂質クラスの決定
JoVE Journal
Ambiente
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JoVE Journal Ambiente
Determination of Total Lipid and Lipid Classes in Marine Samples
DOI:

14:59 min

December 11, 2021

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Capítulos

  • 00:04Introduction
  • 00:32Filtration Procedure for Seawater Dissolved and Particulate Lipids
  • 01:53Liquid-Liquid Extraction of Seawater or Liquid Samples
  • 03:23Extraction Protocol for Solids (Modified Folch et al. Extraction)
  • 06:17Developing and Conditioning Sequences for Separation of Aquatic Lipid Classes by Rod TLC
  • 10:11FAME Derivatization with H2SO4 in MeOH
  • 13:17Representative Results
  • 14:30Conclusion

Summary

Tadução automática

このプロトコルは、海水および生物学的標本中の脂質の決定のためのものです。濾液中の脂質は、固形物の場合にはクロロホルムまたはクロロホルムとメタノールの混合物で抽出される。脂質クラスは、炎イオン化検出を用いたロッド薄層クロマトグラフィーで測定され、その合計が総脂質含有量を示します。

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