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Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking
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Biologia
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JoVE Journal Biologia
Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

Analysis of Motility Patterns of Stentor During and After Oral Apparatus Regeneration Using Cell Tracking

DOI:
10.3791/62352-v

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07:17 min

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April 26, 2021

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, , , ,
1W. M. Keck Science Department,Scripps, Pitzer, and Claremont McKenna of The Claremont Colleges, 2W. M. Keck Science Department,Pitzer College, 3W. M. Keck Science Department,Scripps College, 4Department of Biochemistry and Biophysics, School of Medicine,University of California at San Francisco

Capítulos

  • 00:05Introduction
  • 01:03Sample Preparation
  • 02:15Visible Light Microscopy Time-Lapse
  • 02:56Cell Tracking
  • 03:52Trace Verification
  • 04:26Data Visualization
  • 05:14Results: Motility Patterns of Stentor During and After Oral Apparatus
  • 06:34Conclusion

Summary

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We present a protocol for the characterization of motility and behavior of a population of hundred micron- to millimeter-sized cells using brightfield microscopy and cell tracking. This assay reveals that Stentor coeruleus transitions through four behaviorally distinct phases when regenerating a lost oral apparatus.

Tags

Keywords: Stentor CoeruleusMotility PatternsOral Apparatus RegenerationCell TrackingUnicellular RegenerationFunctional RegenerationSynchronized RegenerationMotility QuantificationSilicone SpacerSample Chambers10% Sucrose2% UreaTime-lapse MicroscopyImage CalibrationCell Tracking Scripts

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