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薄层色谱分析分枝杆菌的脂质成分
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Analysis of the Lipid Composition of Mycobacteria by Thin Layer Chromatography
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薄层色谱分析分枝杆菌的脂质成分

DOI:
10.3791/62368-v

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07:42 min

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April 16, 2021

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, ,
1Departament de Genètica i de Microbiologia, Facultat de Biociències,Universitat Autònoma de Barcelona

Capítulos

  • 00:05Introduction
  • 00:33Extraction of The Total Non-Covalent-Linked Lipids from Mycobacteria
  • 02:13Mycolic Acid Extraction by Acid Methanolysis
  • 03:26Thin Layer Chromatography (TLC) Analysis
  • 04:57Results: TLC Analysis of the Lipid Composition in Different Species of Mycobacteria
  • 07:15Conclusion

Summary

Tadução automática

Tadução automática

提出一个协议，以提取各种分枝杆菌细胞壁的总脂质含量。此外，还显示了不同类型的肌酸的萃取和分析协议。还提供了一个薄层色谱协议来监测这些分形化合物。

Tags

MycobacteriaLipidsThin Layer ChromatographyExtractionChloroformMethanolMycolic AcidN-hexaneTLC

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