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超快力钳谱分析合成性肌球蛋白的机械酶学特性
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Biologia
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JoVE Journal Biologia
Dissecting Mechanoenzymatic Properties of Processive Myosins with Ultrafast Force-Clamp Spectroscopy
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超快力钳谱分析合成性肌球蛋白的机械酶学特性

DOI:
10.3791/62388-v

•

09:38 min

•

July 01, 2021

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, , ,
1National Institute of Optics, National Research Council, 2LENS, European Laboratory for Non-Linear Spectroscopy, 3Physics Department, University of Florence

Capítulos

  • 00:04Introduction
  • 00:47Optical Setup
  • 01:22Silica Beads Preparation
  • 03:24Trap Position, Power, and Stiffness Calibration
  • 04:53Sample Assembly
  • 05:54Sample Measurement and Actin‐Myosin Contact Establishment
  • 07:37Results: Position Recording and Force Inversion
  • 08:53Conclusion

Summary

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这里介绍的是在合成肌球蛋白-5电机上进行超快力钳实验的综合协议，可以很容易地扩展到其他类别的加工电机的研究。该协议详细说明了所有必要的步骤，从实验设备的设置到样品制备，数据采集和分析。

Tags

Mechanoenzymatic PropertiesProcessive MyosinsUltrafast Force-clamp SpectroscopyLaser TweezersChemomechanicsMyosin MotorsForce ProductionForce DirectionalityProcessive MotorsKinesinsDyneinsForce RegulationAcousto-optic DeflectorsIrisObjectiveLaser BeamSilica BeadsPentyl AcetateAcetoneNitrocelluloseEthanolGlass CoverslipNitrogen FlowDouble-sided Tape

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