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1Department of Electrical and Computer Engineering,Boston University, 2Boston University Photonics Center,Boston University, 3Department of Biomedical Sciences and Pathobiology, Virginia-Maryland College of Veterinary Medicine,Virginia Polytechnic Institute and State University, 4Department of Biomedical Engineering,Boston University, 5Department of Chemistry,Boston University
Capítulos
- 00:04Introduction
- 00:38Deuterium (D2O) Incorporation Treatment in the Presence of Antibiotics
- 03:50Preparation of Bacteria in Urine Environment and Blood Environment
- 05:42Stimulated Raman Scattering (SRS) Imaging of D2O Metabolic Incorporation in a Single Bacterium
- 08:44Results: The Influence of Deuterium Incorporation on Bacterial Growth
- 11:14Conclusion
Detta protokoll presenterar snabb testning av antimikrobiell mottaglighet (AST) inom 2,5 timmar genom encellsstimulerad Raman-spridningsavbildning av D2O-metabolism. Denna metod gäller bakterier i urin- eller helblodsmiljön, vilket är transformativt för snabb encellig fenotypisk AST i kliniken.
