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Tools for the Real-Time Assessment of a Pseudomonas aeruginosa Infection Model
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Capítulos
- 00:05Introduction
- 00:34Synthetic Cystic Fibrosis Medium (SCFM2) Preparation
- 01:03Bacterial Cell Suspension Preparation
- 02:36Pseudomonas aeruginosa Aggregate Visualization and Isolation
- 04:48Image Analysis
- 06:15Results: Analysis of Pseudomonas aeruginosa Aggregates
- 07:10Conclusion
Synthetic cystic fibrosis sputum medium (SCFM2) can be utilized in combination with both confocal laser scanning microscopy and fluorescence-activated cell sorting to observe bacterial aggregates at high resolution. This paper details methods to assess aggregate populations during antimicrobial treatment as a platform for future studies.
