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Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry
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Bioquímica
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JoVE Journal Bioquímica
Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

Profiling Volatile Compounds in Blackcurrant Fruit using Headspace Solid-Phase Microextraction Coupled to Gas Chromatography-Mass Spectrometry

DOI:
10.3791/62421-v

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05:29 min

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June 09, 2021

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, ,
1Departamento de Biología Molecular y Bioquímica, Campus de Teatinos,Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga-Consejo Superior de Investigaciones Científicas

Capítulos

  • 00:05Introduction
  • 00:42Sample Preparation
  • 01:17Headspace Solid-phase Microextraction (HS-SPME) Coupled with Gas Chromatography-mass Spectrometry (GC-MS) Data Acquisition
  • 02:23Analysis of GC-MS Profile Chromatograms: VOC Identification and Semi-quantification
  • 03:20Results: Analysis of HS-SPME/GC-MS Chromatograms, PCA, and Relative VOC Content in Blackcurrant Fruits
  • 04:48Conclusion

Summary

Tadução automática

Tadução automática

A headspace solid-phase microextraction-gas-chromatography platform is described here for fast, reliable, and semi-automated volatile identification and quantification in ripe blackcurrant fruits. This technique can be used to increase knowledge about fruit aroma and to select cultivars with enhanced flavor for the purpose of breeding.

Tags

Keywords: Volatile CompoundsBlackcurrant FruitHeadspace Solid-phase MicroextractionGas Chromatography-mass SpectrometryFruit AromaBreeding ProgramsSemi-automated TechniqueBerry CropsSodium Chloride SolutionCentrifugationInternal StandardGC-MS Auto SamplerHeadspace VialSPME DeviceVolatile DesorptionGC-MS AnalysisRetention TimeMass SpectraKovats Linear Retention IndexPeak AreaInternal StandardBatch Effect Correction

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